Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.230213 |
Chromosome: | chromosome_17 |
Location: | 1621336 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g708000 | FAP260 | Flagellar Associated Protein, membrane-associated | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTCTTCAGCGTCAGGTACGAGTACGTGTAG |
Internal bar code: | ATTTACGCCGTTTTGCCAGAGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1061 |
LEAP-Seq percent confirming: | 99.5725 |
LEAP-Seq n confirming: | 4658 |
LEAP-Seq n nonconfirming: | 20 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCTCAACAGTCTGTGGCTGA |
Suggested primer 2: | AAAATAGGATTTGGGGTCGC |