| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230217 |
| Chromosome: | chromosome 10 |
| Location: | 2379537 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435250 | NIC13 | (1 of 1) 6.3.5.1 - NAD(+) synthase (glutamine-hydrolyzing) / Nicotinamide adenine dinucleotide synthetase (glutamine); NAD+ synthase (glutamine-hydrolyzing) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCGTGACGCCTTGCCTCTTGGACTTGC |
| Internal bar code: | AGGGTGAAGCACGGAACTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 555 |
| LEAP-Seq percent confirming: | 71.8072 |
| LEAP-Seq n confirming: | 596 |
| LEAP-Seq n nonconfirming: | 234 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCCCACCATCATCAGAGG |
| Suggested primer 2: | CAGGCGAGCGGAAGTTATAG |