| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230218 |
| Chromosome: | chromosome 5 |
| Location: | 3323926 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240500 | (1 of 1) PTHR24375:SF140 - ZINC FINGER WITH UFM1-SPECIFIC PEPTIDASE DOMAIN PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAGCCAGGCAGGCAGGCAGGCAGCCAGGC |
| Internal bar code: | GACGTATTCCGCAGTGGGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 96.9325 |
| LEAP-Seq n confirming: | 158 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTGAAGGAGCGTGAAG |
| Suggested primer 2: | AAACAAAGCGCAAGGACAGT |