| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230236 |
| Chromosome: | chromosome 4 |
| Location: | 1732956 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212401 | UBC15 | (1 of 2) K10586 - baculoviral IAP repeat-containing protein 6 (apollon) (BIRC6, BRUCE); E2 Ubiquitin conjugating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTAAGGAAGTGGTAGGCGGGTAGAACG |
| Internal bar code: | TCGCAATATACTAACAGTTCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 262 |
| LEAP-Seq percent confirming: | 99.7191 |
| LEAP-Seq n confirming: | 1775 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACCGCTTATCCTACACC |
| Suggested primer 2: | AGATGGTTCCCTCCTGACCT |