| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230269 |
| Chromosome: | chromosome 7 |
| Location: | 3896817 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339100 | (1 of 4) PTHR24361//PTHR24361:SF411 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCTGGCCCAGCCCTACGTGCGCCGCCAC |
| Internal bar code: | ACATTGATCTGACTCCGTTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 99.2398 |
| LEAP-Seq n confirming: | 6397 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCTAACCCTATGTGGGCT |
| Suggested primer 2: | CATTGCCTCCCACTTCCTTA |