| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230278 |
| Chromosome: | chromosome 14 |
| Location: | 3355854 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630125 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCAGTCAGCCAGCAAGCCCAAGGTCCC |
| Internal bar code: | TAAACTATAGGGGGAGTGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 124 |
| LEAP-Seq percent confirming: | 92.233 |
| LEAP-Seq n confirming: | 95 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTTGAGGCATTCTGTAT |
| Suggested primer 2: | AACGCTGATTCTGTGCTGTG |