| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230281 |
| Chromosome: | chromosome 11 |
| Location: | 781925 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467636 | (1 of 66) 2.7.12.1 - Dual-specificity kinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCTCATCAGACACAGCAGCATGAACACG |
| Internal bar code: | TGGCTCGAACATGGTGTGCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 422 |
| LEAP-Seq percent confirming: | 99.8638 |
| LEAP-Seq n confirming: | 1466 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGACCGAAGTCACACAGC |
| Suggested primer 2: | ATTCCCTTCATGCGTTCTGT |