| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230281 |
| Chromosome: | chromosome 16 |
| Location: | 805421 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647550 | HEL60 | (1 of 3) PTHR24031//PTHR24031:SF232 - RNA HELICASE // ATP-DEPENDENT RNA HELICASE TDRD12-RELATED; DEAD box ATP-dependent RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACATGAACCGATGAACGCAGCGCACAAA |
| Internal bar code: | GGACGTAGCTTGGTGTGGGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 371 |
| LEAP-Seq percent confirming: | 99.6296 |
| LEAP-Seq n confirming: | 269 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGGACTGCAGAGATGGTG |
| Suggested primer 2: | ATAGCGAGGCTGACTCCTGA |