| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230305 |
| Chromosome: | chromosome 11 |
| Location: | 1057039 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467673 | PDE24 | (1 of 17) 3.1.4.53 - 3',5'-cyclic-AMP phosphodiesterase / cAMP-specific phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGAATTGACGCACCGCACAAACACACGT |
| Internal bar code: | ATTCGGACGGCCCACCACGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 778 |
| LEAP-Seq percent confirming: | 99.7715 |
| LEAP-Seq n confirming: | 14844 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGAGACATGCTGGGGAAC |
| Suggested primer 2: | GCAAACGACATGGAGTCACA |