| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230309 |
| Chromosome: | chromosome_12 |
| Location: | 3924448 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g516150 | NAT27 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGTTCCCCATTCGCGGTGTTCGAAATTCA |
| Internal bar code: | GCAACATCGGTGAAATACCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 335 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACAAAATGCCCTAGCAC |
| Suggested primer 2: | GTGATTGGTGAACATGACGC |