| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230312 |
| Chromosome: | chromosome 12 |
| Location: | 3550020 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g513400 | EBG1 | Endo-1%252C3(4)-beta-glucanase; (1 of 1) 3.2.1.6 - Endo-1,3(4)-beta-glucanase / Laminarinase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGGCAACCGCGTGTACAATGACCACCAC |
| Internal bar code: | GAAGCGTATATTTGGGGTCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 430 |
| LEAP-Seq percent confirming: | 98.7662 |
| LEAP-Seq n confirming: | 7845 |
| LEAP-Seq n nonconfirming: | 98 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTGGATGGCGTGGATGTA |
| Suggested primer 2: | CATTGTGATTGCCGTTTGAC |