| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230343 |
| Chromosome: | chromosome 14 |
| Location: | 1081594 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g614850 | FAP33,ANK17 | Flagellar Associated Protein 33; (1 of 78) IPR002110//IPR020683 - Ankyrin repeat // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCATCAAGGTACGGCAAGTGTACGGC |
| Internal bar code: | GTTGTGTGGCGGGCAGTCGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1036 |
| LEAP-Seq percent confirming: | 97.381 |
| LEAP-Seq n confirming: | 8775 |
| LEAP-Seq n nonconfirming: | 236 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGAGCCTACCAGAAGACG |
| Suggested primer 2: | GATGTGTCACAGACGCTGCT |