| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230344 |
| Chromosome: | chromosome 1 |
| Location: | 603325 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003487 | (1 of 1) K13106 - pre-mRNA-splicing factor CWC26 (BUD13, CWC26) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCACTGCCCAGACCCTCAATTCACACCA |
| Internal bar code: | CGTTACTCAACGTTATTAGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 405 |
| LEAP-Seq percent confirming: | 98.5263 |
| LEAP-Seq n confirming: | 468 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTGCTACTGCTGCTACT |
| Suggested primer 2: | CGAACGGTTTGTGTGATACG |