Insertion junction: LMJ.RY0402.230374_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.230374
Chromosome:chromosome 4
Location:2894507
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g224500 (1 of 1) 3.6.3.17//3.6.3.44 - Monosaccharide-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTGCGAGGGTGGCTGTCCCCACATTTCG
Internal bar code:CATCAATATGAGTGTGACGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1034
LEAP-Seq percent confirming:87.4525
LEAP-Seq n confirming:460
LEAP-Seq n nonconfirming:66
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AACGATTTGTTTGGGCTGAC
Suggested primer 2:GTTGCTGCTGCTATTCCTCC