| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230380 |
| Chromosome: | chromosome 3 |
| Location: | 8202354 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200050 | CAL1 | (1 of 2) PF13202//PF13833 - EF hand (EF-hand_5) // EF-hand domain pair (EF-hand_8); Calpain family cysteine protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGGATCCCTAGAGCTTAAATGAAGGCA |
| Internal bar code: | CGGGGAGGACAATTATATGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 98.0877 |
| LEAP-Seq n confirming: | 5437 |
| LEAP-Seq n nonconfirming: | 106 |
| LEAP-Seq n unique pos: | 136 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATGGGAGACACGCAAAC |
| Suggested primer 2: | GTAGATGCGGAGCTTTGGAG |