Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.230394 |
Chromosome: | chromosome_17 |
Location: | 2789450 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre17.g718750 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGTCATGGGTGGCATTGTGCAGACTCCAAC |
Internal bar code: | CAGAGGTCACTTGCAGGAAGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 134 |
LEAP-Seq percent confirming: | 91.8367 |
LEAP-Seq n confirming: | 45 |
LEAP-Seq n nonconfirming: | 4 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGGGGTCACTGTTTCTTGTT |
Suggested primer 2: | CTCACCATCTGTGTGATGGC |