| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230397 |
| Chromosome: | chromosome 7 |
| Location: | 4606998 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344150 | (1 of 1) PTHR21314//PTHR21314:SF0 - UNCHARACTERIZED // UPF0553 PROTEIN C9ORF64 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTCAAACAGAGCTGTTGCGTTCTCACAT |
| Internal bar code: | TGTAGGGGGAGCCTTCTTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 506 |
| LEAP-Seq percent confirming: | 99.5715 |
| LEAP-Seq n confirming: | 1162 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTCGGGTACATAACCTC |
| Suggested primer 2: | CGTCGACAGGCTAGAGTTCC |