| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230403 |
| Chromosome: | chromosome 6 |
| Location: | 5945290 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288700 | GYD1 | Glycolate dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGTGTCGGGAGTGCACGACAGTAAGCG |
| Internal bar code: | GTGCGCCCTCGCAAAGCACTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 266 |
| LEAP-Seq percent confirming: | 99.7213 |
| LEAP-Seq n confirming: | 1789 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACTGAACACCCCTTGTG |
| Suggested primer 2: | CATCTTCTTGGAGGAGCAGG |