| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230457 |
| Chromosome: | chromosome 5 |
| Location: | 3172048 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239550 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAGCGCGCTTTCACGCACTTCTACGCAC |
| Internal bar code: | CCTTTACGTTGGCTTTCCCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1094 |
| LEAP-Seq percent confirming: | 99.3713 |
| LEAP-Seq n confirming: | 2845 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCCTAAGAGATGGTGGTC |
| Suggested primer 2: | AGGACAGGGTGCATACTTGG |