| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230459 |
| Chromosome: | chromosome 7 |
| Location: | 20640 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g312050 | (1 of 1) 1.5.3.1//1.5.3.7 - Sarcosine oxidase // L-pipecolate oxidase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGCTAGTGCATGGCAAGTCTGCCGTC |
| Internal bar code: | AGAAATACTCGTATCCATCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 760 |
| LEAP-Seq percent confirming: | 99.4668 |
| LEAP-Seq n confirming: | 12311 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAAACAACATGGAAACGC |
| Suggested primer 2: | ATCACCAACCCTGGCAGTAG |