| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230486 |
| Chromosome: | chromosome 9 |
| Location: | 2936089 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387950 | (1 of 40) PTHR23257//PTHR23257:SF513 - SERINE-THREONINE PROTEIN KINASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCCCCCGTGAGCATCGCCCAGATGCAC |
| Internal bar code: | CTTAGTCTGTGAAAGAGTGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 705 |
| LEAP-Seq percent confirming: | 99.5736 |
| LEAP-Seq n confirming: | 10041 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGCACCCTATCGTAAAGC |
| Suggested primer 2: | CCCTGAAATTCTGGCATGTT |