| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230489 |
| Chromosome: | chromosome 12 |
| Location: | 5737168 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532950 | (1 of 4) PF00060//PF00497 - Ligand-gated ion channel (Lig_chan) // Bacterial extracellular solute-binding proteins, family 3 (SBP_bac_3); Ligand-gated ion channel | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAAGCCTGGTGGGGTGAGCGAGCGGGTG |
| Internal bar code: | TCCAGGCCGGTGATAAATAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 952 |
| LEAP-Seq percent confirming: | 98.8827 |
| LEAP-Seq n confirming: | 1770 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTCCGTGTTTTTCCAGC |
| Suggested primer 2: | TCCACACGCACATAACCCTA |