| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230496 |
| Chromosome: | chromosome 5 |
| Location: | 2517865 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g235800 | FBB19 | Flagellar/basal body protein 19 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACACTGGAGATGGGCGCGTCGTAGCGCGG |
| Internal bar code: | TGATATTGGGAAGTTAGAAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 627 |
| LEAP-Seq percent confirming: | 99.0516 |
| LEAP-Seq n confirming: | 940 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGGAGGAAATCGGTGAG |
| Suggested primer 2: | AAGAGTGAGATGGCAAGCGT |