| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230506 |
| Chromosome: | chromosome 14 |
| Location: | 1215814 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616100 | FKB11,FKB53 | (1 of 1) K14826 - FK506-binding nuclear protein [EC:5.2.1.8] (FPR3_4); Peptidyl-prolyl cis-trans isomerase, FKBP-type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGAGGACGGCAAGGAAGCGTTATGGCA |
| Internal bar code: | GGTTTTTGACTTAGGTTGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 398 |
| LEAP-Seq percent confirming: | 96.5347 |
| LEAP-Seq n confirming: | 390 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGATGAGAGATGGGGA |
| Suggested primer 2: | GACTGAGTGGTAAGCAGGGC |