| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230522 |
| Chromosome: | chromosome 4 |
| Location: | 1740798 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g212300 | PPP17 | (1 of 1) PTHR13832:SF224 - PROTEIN PHOSPHATASE 2C 70; Phosphoprotein phosphatase 2C-related | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAGGCACACGGCGACGCCAAAGGAATC |
| Internal bar code: | GTTGGGTCTGTGCGTCTCCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1058 |
| LEAP-Seq percent confirming: | 99.4191 |
| LEAP-Seq n confirming: | 6846 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAAACGGTTGGTTCAGT |
| Suggested primer 2: | CCAACAGCTCCTCTACGGAC |