| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230538 |
| Chromosome: | chromosome 9 |
| Location: | 7519309 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414050 | Pontin,RVB1,RUVBL1 | Nucleoside-triphosphatase RuvB-like protein; (1 of 1) K04499 - RuvB-like protein 1 (pontin 52) (RUVBL1, RVB1, INO80H) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGGGTAGCAGCATCCTCTCCTGCACACT |
| Internal bar code: | TGTAAAGACAAGGCCTGGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 60 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTGCGATCCTACTTGTGT |
| Suggested primer 2: | TTGAGCATGGGGTAAGAACC |