| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230593 |
| Chromosome: | chromosome 16 |
| Location: | 5135629 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g683700 | (1 of 10) PF03798 - TLC domain (TRAM_LAG1_CLN8) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCAGCCCGGTGAGCGCCACTTGGAAGAT |
| Internal bar code: | GGCGTCGAAACGATGAGTGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 99.7312 |
| LEAP-Seq n confirming: | 371 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGCTCATCTCCACCAC |
| Suggested primer 2: | ATACACCACAAGCCCTCGAC |