Insertion junction: LMJ.RY0402.230616_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.230616
Chromosome:chromosome 1
Location:4774830
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g033000 FAP334 (1 of 2) IPR000157//IPR016024 - Toll/interleukin-1 receptor homology (TIR) domain // Armadillo-type fold; Flagellar Associated Protein 334 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGTCCAGCAGAGGTTTCATCCGGCAGCG
Internal bar code:CTTCGCAGACCAACTTCAGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:173
LEAP-Seq percent confirming:88.454
LEAP-Seq n confirming:452
LEAP-Seq n nonconfirming:59
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:AGCCCAAGTAGACCAGACCA
Suggested primer 2:GTTCCTGCACAATGCATCAC