| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230621 |
| Chromosome: | chromosome 16 |
| Location: | 6621663 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671950 | (1 of 7) IPR000305 - GIY-YIG nuclease superfamily | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCAACGGCAACCGGCGTGAGTGGACAGC |
| Internal bar code: | CGAGGATCCGGAACAGATGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 308 |
| LEAP-Seq percent confirming: | 96.8981 |
| LEAP-Seq n confirming: | 656 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACGCCATCTACGACGAG |
| Suggested primer 2: | GTCCATATCCCTCCCTTGGT |