| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230625 |
| Chromosome: | chromosome 7 |
| Location: | 4003516 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339900 | MAPKKK5 | Mitogen-Activated Protein Kinase Kinase Kinase; (1 of 18) 2.7.10.2//2.7.11.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Non-specific serine/threonine protein kinase / Threonine-specific protein kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGCCCAACCCAGATCGTGGTTGCTGTG |
| Internal bar code: | CGAATCGAGGGAGCGCGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 665 |
| LEAP-Seq percent confirming: | 99.3779 |
| LEAP-Seq n confirming: | 639 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACACTTTTCACCAGCGAG |
| Suggested primer 2: | AGAGCGCATACTCCGTCAGT |