Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.230646 |
Chromosome: | chromosome_8 |
Location: | 487135 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g358800 | antisense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGGGCAAGGAGAGGCACAGTTCCATTTGTG |
Internal bar code: | CTCGTACTGCACCGAAGAGCGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 555 |
LEAP-Seq percent confirming: | 93.9799 |
LEAP-Seq n confirming: | 281 |
LEAP-Seq n nonconfirming: | 18 |
LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATTCACACCGCAAAACACAA |
Suggested primer 2: | CGCAAAGGCTAGGTCAACTC |