Insertion junction: LMJ.RY0402.230659_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.230659
Chromosome:chromosome 16
Location:4854495
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre16.g686050 (1 of 1) PF05277 - Protein of unknown function (DUF726) (DUF726) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGGGGGGGGGATAGCCATCCATGGGATGG
Internal bar code:AGCAGGTGGAGCACAAGGAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:323
LEAP-Seq percent confirming:98.913
LEAP-Seq n confirming:91
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GAGAGGCGGGTATTGTGTGT
Suggested primer 2:CGCACATCTATACACACGCC