| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230660 |
| Chromosome: | chromosome_1 |
| Location: | 1774382 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009550 | NAT6 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGAAGCGGTAGACGGGGGCAGGTGCGAGG |
| Internal bar code: | ACGGAGCTCCAGATAACATTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 444 |
| LEAP-Seq percent confirming: | 96.6292 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGTACGTGTACGGCATGT |
| Suggested primer 2: | GAGGAGTTCTTCAAGGTGCG |