| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230675 |
| Chromosome: | chromosome 6 |
| Location: | 5113403 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281100 | MUT9,ROC94 | (1 of 2) PTHR11909:SF146 - CASEIN KINASE-LIKE PROTEIN; Serine/threonine protein kinase, transcription repression | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGCTCGGTCGCCGGGTGATACCCGCG |
| Internal bar code: | TGGCCCAAAGCTGCTGGACCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1019 |
| LEAP-Seq percent confirming: | 99.4957 |
| LEAP-Seq n confirming: | 12825 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACAGCCCACCATGATGTAG |
| Suggested primer 2: | AATCGACCTTTGTGCTGCTT |