Insertion junction: LMJ.RY0402.230688_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.230688
Chromosome:chromosome 2
Location:1740108
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g086350 3'UTR_intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGCGCCATCGGGCGCGCGCGGTAGGTCCC
Internal bar code:GTCGGTGGACCGTGGTGAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:40
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:125
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATTGCTAGGCACCTGGTTTG
Suggested primer 2:AACTTGTGCACCTACCCCAG