| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.230693 |
| Chromosome: | chromosome 16 |
| Location: | 6374372 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g673900 | RPC3 | DNA-directed RNA polymerase III; (1 of 1) K03023 - DNA-directed RNA polymerase III subunit RPC3 (RPC3, POLR3C) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGGCGGCACGGCCCTGGCAGGTGGAGATA |
| Internal bar code: | CCTCAGGCGCCACGACCAACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 99.0148 |
| LEAP-Seq n confirming: | 804 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTAGCAGCGTCATTTCTC |
| Suggested primer 2: | CATTGACAACCGGACAACAG |