Insertion junction: LMJ.RY0402.230694_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.230694
Chromosome:chromosome 6
Location:1454853
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g259401 SMM16 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 2) PTHR10108//PTHR10108:SF813 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCCCACCTCCCCACCCCCCAAACACACT
Internal bar code:TCAGGCGGCAGGATAGGGCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:341
LEAP-Seq percent confirming:99.5025
LEAP-Seq n confirming:2400
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CAACCCCAACATCCAGTACC
Suggested primer 2:GCACAAACACCAGCTTCTCA