| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.230694 |
| Chromosome: | chromosome 14 |
| Location: | 468460 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTGATGCAGCACGAATCAAAGAACCAG |
| Internal bar code: | CAAGTGAGGGATCAACGTGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 98.8532 |
| LEAP-Seq n confirming: | 431 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTCCTCTGGAAAGTGTGA |
| Suggested primer 2: | GCCATTGCAGAACTTCCATC |