| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230704 |
| Chromosome: | chromosome 16 |
| Location: | 4161728 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g669000 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGCCTGCGCCAGCGCCGCATCCGCCTC |
| Internal bar code: | GGCGGCACCGCGTTACTGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1101 |
| LEAP-Seq percent confirming: | 99.6893 |
| LEAP-Seq n confirming: | 4171 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGCCGCTTATCTTGAGAC |
| Suggested primer 2: | TTGAAGTGGTTGAGGTGCTG |