| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230728 |
| Chromosome: | chromosome 2 |
| Location: | 4027018 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097200 | SRR14 | Scavenger receptor cysteine rich (SRCR) protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACGCTTCAGGTAGGCACCATACCTCAGT |
| Internal bar code: | TTTCGGATCAATCTGAAGTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 659 |
| LEAP-Seq percent confirming: | 99.5596 |
| LEAP-Seq n confirming: | 2487 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGACTGTGGGTGGACAGTG |
| Suggested primer 2: | GAGCCCACTGTCTTTCTTGC |