Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.230742 |
Chromosome: | chromosome 16 |
Location: | 1841291 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g655450 | (1 of 1) PF14510 - ABC-transporter extracellular N-terminal (ABC_trans_N) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGTTCAGCACACCCTGCTAGCAACGCG |
Internal bar code: | GACCAGCCCGGACGAATGGAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 600 |
LEAP-Seq percent confirming: | 95.9701 |
LEAP-Seq n confirming: | 643 |
LEAP-Seq n nonconfirming: | 27 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AAAACTTGGCTTGGTCATGG |
Suggested primer 2: | CGGGCCACAAAACTTACCTA |