Insertion junction: LMJ.RY0402.230745_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.230745
Chromosome:chromosome 2
Location:1451291
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g083750 ROC75 (1 of 1) IPR006447//IPR009057//IPR017930 - Myb domain, plants // Homeodomain-like // Myb domain; Rhythm Of Chloroplast 75 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCTGCTGCTGCAGTAGCTGCTGCAGCT
Internal bar code:CCCCGGGAGATGACCTACGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:147
LEAP-Seq percent confirming:99.3865
LEAP-Seq n confirming:486
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAGACATCGACATGGACGAC
Suggested primer 2:GTTGCTGTTGCTGTTGCTGT