| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230754 |
| Chromosome: | chromosome 9 |
| Location: | 1713088 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396750 | CSE18 | Predicted protein of CSE family; (1 of 1) IPR000601//IPR022409 - PKD domain // PKD/Chitinase domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGACTGGTGCCGCATGCCATCTCAGCGC |
| Internal bar code: | GTATACAATTCCGGAGTTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 99.7903 |
| LEAP-Seq n confirming: | 2855 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGATGCGTGTTGATACCG |
| Suggested primer 2: | TCTCCTTCCCAACCACACAC |