Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.230754 |
Chromosome: | chromosome_9 |
Location: | 1713096 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g396750 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGCAGATACAGCACGGCGCCTTACCACTA |
Internal bar code: | CCGTGCAGCGACCGTTTACCGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 471 |
LEAP-Seq percent confirming: | 99.375 |
LEAP-Seq n confirming: | 1908 |
LEAP-Seq n nonconfirming: | 12 |
LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTGATGCGTGTTGATACCG |
Suggested primer 2: | TCTCCTTCCCAACCACACAC |