| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230804 |
| Chromosome: | chromosome 3 |
| Location: | 4602822 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g177053 | (1 of 2) K17263 - cullin-associated NEDD8-dissociated protein 1 (CAND1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCCGATCTACAGTCTTCAGCGCATCCTG |
| Internal bar code: | GATCACAAAATTGTCGTCCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 99.7321 |
| LEAP-Seq n confirming: | 1117 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCGACTTGGTAAGGCA |
| Suggested primer 2: | TTTTCTCCCCCTCTATGCCT |