| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230822 |
| Chromosome: | chromosome_9 |
| Location: | 4477986 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g395769 | FAP177 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CTGATCGCGGTGCTGAGTTGCCGAGTTTGT |
| Internal bar code: | TTGGTCCGACCGAGGGGACTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 99.2825 |
| LEAP-Seq n confirming: | 5673 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGACGTGAGTTGCTTTGA |
| Suggested primer 2: | ACTTGTCCATGCGTAGTCCC |