| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.230824 |
| Chromosome: | chromosome 6 |
| Location: | 5877091 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g288050 | MGTE1,MGTE | Mg2+/divalent cation transporter; (1 of 2) PF01769 - Divalent cation transporter (MgtE) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTACGATCCCTTCCGGCCCGCTCACCCACC |
| Internal bar code: | GTTTCCGCTGGCGGGTGGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 29 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCATGTGACAGCTCTCCT |
| Suggested primer 2: | CGGCTTACAAGAACAGGCTC |