| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.230841 |
| Chromosome: | chromosome 6 |
| Location: | 2117709 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g265550 | CDPK12 | (1 of 1) PF07714//PF13202 - Protein tyrosine kinase (Pkinase_Tyr) // EF hand (EF-hand_5); Calcium/calmodulin-dependent protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGGAGGCATCGTTTTTTGTGGTCCTT |
| Internal bar code: | CGTACGTAGGAGATAGCCCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 91 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGCCCAGGACTTCAAGA |
| Suggested primer 2: | CTGGAGTTCTACGAGAGCCG |