Insertion junction: LMJ.RY0402.230847_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.230847
Chromosome:chromosome 10
Location:1940147
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g432150 (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCGGCTTCGCACCTGCCATTCGTGTTCA
Internal bar code:CAGTACAAACGGGTGTCAGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:570
LEAP-Seq percent confirming:96.5251
LEAP-Seq n confirming:250
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGAATCAGACGGACCAAA
Suggested primer 2:ACACAGCTGCTACCAGGGAC