Insertion junction: LMJ.RY0402.230865_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.230865
Chromosome:chromosome 12
Location:5916795
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g534151 EEP9 (1 of 3) PTHR22748:SF4 - DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE 2; endonuclease/exonuclease/phosphatase family protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGTGGCACAGGACTCGCGTCCGCTGCAA
Internal bar code:GCCCGTGTTCGTATTGTCGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:335
LEAP-Seq percent confirming:70.1493
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCTTTAAGAGACGTGTGC
Suggested primer 2:GAAAGCAAAGGAGGCGAAG